| Cell Line Species/Tissue: |
mouse
/
mast cell |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI, 10% FCS |
Percent Serum (%):
|
10 |
Reporter System:
|
G418 |
Plasmid Purification Method:
|
Silica slurry |
Plate Format:
|
60-mm dish |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1 |
Amount of Enhancer (µl):
|
8 |
Amount of Reagent (µl):
|
25 |
Complex Incubation on Cells (hrs):
|
48 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
>60 colonies |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Split cells the day prior to transfection. Also tested SuperFect and obtained 53 colonies. |
| References: |
| |