Transfection Cell Database - Detailed View


Cell Line: J82

Cell Line Species/Tissue: human / bladder, transitional-cell carcinoma 
Transfection Reagent: Effectene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
 
Reporter System:
GFP 
Plasmid Purification Method:
EndoFree Plasmid Kit 
Plate Format:
6-well plate 
Number of Cells:
 
Percent Confluence(%):
70 
Amount of Nucleic Acid (µg):
0.4 
Amount of Enhancer (µl):
3.2 
Amount of Reagent (µl):
10 
Complex Incubation on Cells (hrs):
24 
Analysis Performed Post-Transfection (hrs):
48 
Transfection Efficiency (%):
16% 
 
Any modifications to the protocol?:
 
Notes:
Analysis via FACS.  
References: