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Enzymes for Molecular Biology

Enzymes used for molecular biology applications

Are you looking for enzymes that will drive your work to success? We offer a range of enzymes for molecular biology and life science applications that are high in activity, consistent in quality, reliably pure and backed by the QIAGEN name.

Our enzyme products are engineered to produce the best native, novel or altered enzymes. These are available to science and industry to catalyze a wide range of specific uses in analytical devices and assays that have clinical, forensic and environmental applications.

Browse Enzymes for Molecular Biology
Exonuclease I
Exonuclease I
For removal of single-stranded primers in PCR reactions
StableScriptâ„¢ One-Step RT-qPCR Mix
StableScriptâ„¢ One-Step RT-qPCR Mix
A highly efficient and sensitive RT-qPCR formulation for detection using hydrolysis probes with robust inhibitor resistance
WGS Fragmentation Mix
WGS Fragmentation Mix
For fragmentation, end-repair and A-tailing for NGS library preparation in a single reaction
T4 RNA Ligase 2, Truncated
T4 RNA Ligase 2, Truncated
For specific ligation of the adenylated 5´ end of an adapter to the 3´ end of RNA
ER/A-Tailing Enzyme Mix
ER/A-Tailing Enzyme Mix
For end-repair and dA-tailing in a one-step reaction
Thermolabile UNG
Thermolabile UNG
For removal of uracil from DNA and carry over prevention in PCR
WGS Ligase
WGS Ligase
For NGS adapter ligation
VeraSeqâ„¢ 2.0 High-Fidelity DNA Polymerase
VeraSeqâ„¢ 2.0 High-Fidelity DNA Polymerase
Ultrathermostable polymerase for high-fidelity PCR
VeraSeqâ„¢ ULtra DNA Polymerase
VeraSeqâ„¢ ULtra DNA Polymerase
Ultrathermostable uracil-literate polymerase for high-fidelity PCR
Lambda Exonuclease
Lambda Exonuclease
For conversion of linear double-stranded DNA to single-stranded DNA.
VeraSeqâ„¢ PCR Mix
VeraSeqâ„¢ PCR Mix
Ultrathermostable mastermix for high-fidelity PCR
Endonuclease VIII
Endonuclease VIII
For generating and cleaving AP sites leaving 3ʹ and 5ʹ phosphates
Taq DNA Ligase
Taq DNA Ligase
For joining or ligation of DNA fragments, particularly in DNA sequencing and cloning techniques
T4 RNA Ligase 2
T4 RNA Ligase 2
For the ligation of single-stranded or double-stranded RNA molecules, as well as RNA-DNA hybrids
T4 RNA Ligase 1
T4 RNA Ligase 1
For the ligation of RNA molecules, facilitating the joining of RNA fragments or the addition of RNA adapters
Terminal Deoxynucleotidyl Transferase (TdT)
Terminal Deoxynucleotidyl Transferase (TdT)
For facilitating the labeling of DNA probes and tailing of DNA fragments
Uracil Cleavage System
Uracil Cleavage System
For creating an abasic site with an intact phosphodiester backbone and liberating the deoxyribose sugar
Thermostable Pyrophosphatase
Thermostable Pyrophosphatase
For prevention or removal of inorganic pyrophosphate (PPi) from DNA or RNA synthesis reactions
T4 DNA Ligase
T4 DNA Ligase
For restriction and TA cloning and adapter ligation
Uracil DNA Glycosylase
Uracil DNA Glycosylase
For catalyzing the hydrolysis of the N-glycosylic bond between uracil and the sugar
Thermolabile UDG
Thermolabile UDG
For removal of uracil from DNA and carry over prevention in PCR
T3 DNA Ligase
T3 DNA Ligase
For cloning ligation and nick-sealing in double-stranded DNA
T7 DNA Ligase
T7 DNA Ligase
For cloning ligation and nick-sealing in double-stranded DNA
TaqIT DNA Polymerase
TaqIT DNA Polymerase
For reverse transcription from RNA for first strand cDNA synthesis and RT-PCR
Poly(A) Polymerase
Poly(A) Polymerase
For making polyA tailed RNA and 3’ end RNA labeling
Klenow Fragment
Klenow Fragment
For removal of 3’ overhangs and filling in of 5’ overhangs
E. coli Single-Stranded DNA Binding Protein
E. coli Single-Stranded DNA Binding Protein
For binding and stabilizing single-stranded DNA (ssDNA) during various DNA polymerase-mediated reactions
E. coli Pyrophosphatase
E. coli Pyrophosphatase
For effectively hydrolyzing inorganic pyrophosphate (PPi), helping maintain the enzymatic activity
E. coli DNA Ligase
E. coli DNA Ligase
For cDNA cloning by replacement synthesis
EZ1&2 DNA FFPE Kits
EZ1&2 DNA FFPE Kits
For automated purification of gDNA from FFPE tissues using the EZ1 Advanced XL or EZ2 Connect instrument
End-Repair Mix
End-Repair Mix
For converting DNA with 5′- and/or 3′-protruding ends to 5′-phosphorylated, blunt-ended DNA
T4 Gene 32 Protein
T4 Gene 32 Protein
For increasing PCR amplification and DNA sequencing efficiency
T4 Polynucleotide Kinase
T4 Polynucleotide Kinase
For phosphorylation of the 5’-terminus of polynucleotides with ATP gamma-phosphate
Exonuclease III
Exonuclease III
For directional digestion of nucleic acids and analysis of DNA-protein interactions
Taq-B DNA Polymerase
Taq-B DNA Polymerase
For routine PCR amplification of DNA fragments up to 5 kb
DNA Polymerase I
DNA Polymerase I
For labeling of DNA
T4 DNA Polymerase
T4 DNA Polymerase
For cloning, DNA labeling and recombination applications
Klenow Fragment (3'→5' exo–)
Klenow Fragment (3'→5' exo–)
For fluorescent labeling of DNA and dA/dT tailing for ligation of DNA
RNase Inhibitor
RNase Inhibitor
For use in applications where the presence of RNases may pose a risk to RNA quality
StableScript Reverse Transcriptase
StableScript Reverse Transcriptase
For reverse transcription in one-step RT-qPCR and long-range RT-PCR
EnzScript Reverse Transcriptase
EnzScript Reverse Transcriptase
For reverse transcription from RNA for first strand cDNA synthesis and RT-PCR
Manta 1.0 DNA Polymerase
Manta 1.0 DNA Polymerase
Strong strand-displacement activity for isothermal amplification.
ZipScript and ZipScript WarmX One-Step RT-qPCR Kits
ZipScript and ZipScript WarmX One-Step RT-qPCR Kits
For highly sensitive and specific one-step and multiplex qRT-PCR
Reverse Transcriptase
Reverse Transcriptase
For synthesis of complementary DNA strand in the presence of a primer using either RNA or single-stranded DNA
Reverse Transcription Kit
Reverse Transcription Kit
For synthesis of first-strand cDNA from the total RNA or mRNA templates
RNase A MBG
RNase A MBG
For removal of RNA during the isolation procedures of plasmid and genomic DNA
RNase H MBG
RNase H MBG
For the removal of mRNA after first-strand cDNA synthesis
RNase H
RNase H
For cleaving the RNA strand of DNA:RNA hybrids

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