How is total RNA purified with RNeasy kits free of genomic DNA contamination?
The composition of lysis and binding buffer combined with a special silica matrix ensures a selective binding of RNA, while genomic DNA does not bind. However, further DNA removal may be necessary for RNA applications that are sensitive to very small amounts of DNA (e.g., TaqMan® RT-PCR analysis with a low-abundant target). RNeasy Plus Mini Kit or RNeasy Plus Universal Kit provides convenient RNA purification with effective elimination of genomic DNA.