What factors determine if denatured proteins accumulate underneath, or inside the gel of the MaXtract Low and High Density Tubes?

Generally, this is determined by the density of the denatured proteins and the gel type in the MaXtract Tubes. If proteins show a higher density than the gel, they will accumulate underneath in the organic phase. If they have the same density as the MaXtract gel, they will collect in the gel. The quantity and ratio of phenol:chloroform can also influence the behavior of the proteins.