Can PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue be used for in situ hybridization?

Yes, human tissue samples treated with the PAXgene Tissue System were successfully used for Fluorescence in situ hybridization (FISH) and Chromogenic in situ hybridization (CISH). However, specimens treated with the PAXgene Tissue System are more sensitive to enzyme digestion compared to formalin-fixed samples. Enzyme digestion and other pretreatment steps may need to be optimized.