What are the expected PCR results when using EpiTect Control DNA on untreated or bisulfite-converted DNA?
Expected PCR results when using the EpiTect Control DNA Set in combination with the EpiTect MSP Kit can be found in the table below:
Type of DNA | Primer for unmethylated & unconverted genomic DNA | Primer for unmethylated & bisulfite converted target DNA | Primer for methylated & bisulfite converted target DNA |
Unmethylated & untreated control DNA | PCR product | NO PCR product | NO PCR product |
Unmethylated & bisulfite converted control DNA | NO PCR product | PCR product | NO PCR product |
Methylated & bisulfite converted control DNA | NO PCR product | NO PCR product | PCR product |
No template control | NO PCR product | NO PCR product | NO PCR product |