| Cell Line Species/Tissue: |
Human
/
Macrophages |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI 1640 |
Percent Serum (%):
|
10% fetal bovine serum,2 mM glutamine, 100 U/ml penicillin, |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
|
Percent Confluence(%):
|
no information |
Amount of Nucleic Acid (µg):
|
5 nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
4,5 µl |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
40 hrs |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Macrophages, cultured in 24-well plates, were incubated with the transfection mix containing 5 nM siRNA and
4.5 µl of HiPerFect Reagent in 500 µl of culture medium. |
| References: |
| Aryl Hydrocarbon Receptor- and Calcium-dependent
Induction of the Chemokine CCL1 by the Environmental
Contaminant Benzo[a]pyrene
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 281, NO. 29, pp. 19906–19915, July 21, 2006
Monique NDiaye‡1, Eric Le Ferrec‡2, Dominique Lagadic-Gossmann‡, Se´bastien Corre§, David Gilot‡,
Vale´ rie Lecureur‡, Patricia Monteiro‡1, Claudine Rauch‡, Marie-Dominique Galibert§, and Olivier Fardel‡ |
|