| Cell Line Species/Tissue: |
Mouse
/
embryo stem cells |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
|
Percent Serum (%):
|
10 % FBS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well |
Number of Cells:
|
2x10e5/well |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
1.6~3.2µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
15 µl |
Complex Incubation on Cells (hrs):
|
24 h |
Analysis Performed Post-Transfection (hrs):
|
24 h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
80% |
| |
| Any modifications to the protocol?:
|
| None |
| Notes: |
| Analysis method: real-time quantitative PCR |
| References: |
| |