| Cell Line Species/Tissue: |
Human
/
T cell leukemia |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
10% FCS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well plate |
Number of Cells:
|
200.000 cells/well |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
250;125; 75nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
12µl; 9 µl |
Complex Incubation on Cells (hrs):
|
48h |
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
75% |
| |
| Any modifications to the protocol?:
|
| 200µl that contain cells and complexes were incubated for 6 hours, then 400µl RPMI+S were added |
| Notes: |
| QIAGEN CDC2 siRNA; qRT-PCR Analysis; Protocol modification: 200µl that contain cells and complexes were incubated for 6 hours, then 400µl RPMI+S were added. The combination of 75nM siRNA and 9µl HiPerfect worked best. |
| References: |
| |