PAXgene Blood RNA Tubes (IVD)

For collection of whole blood samples for molecular diagnostic testing

S_1126_9_PAXgene_Blood_RNA_Tube_IVD
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PAXgene Blood RNA Tube (100), IVD

Cat. No. / ID:   762165

100 PAXgene Blood RNA Tubes. This product is sold by BD. Clicking inquire will take you to BD Customer Care.
The PreAnalytiX® PAXgene® Blood RNA Tube (IVD) (Cat. no. 762165) is CE marked for In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on In Vitro Diagnostic Medical Devices (IVDR). The PAXgene Blood RNA Kit (IVDR compliant, Cat No. 762174) is compatible with both versions of the PAXgene Blood RNA Tube (IVD): the CE marked product for in vitro diagnostic use according to the In-vitro Diagnostic Directive 98/79/EC (IVDD) and according to the IVDR.
PAXgene Blood RNA Tubes are intended for in vitro diagnostic testing (IVD) and are to be used in conjunction with the PAXgene Blood RNA Kit. These products are not available in all countries; please inquire.
The PAXgene Blood RNA Tubes (IVD) is intended for in vitro diagnostic use.
Want to try this solution for the first time?
Get in touch with our specialists if you need a quote or if you would like to discuss your project.

Features

  • Coupled with the PAXgene Blood RNA Kit (IVD), constitutes the PAXgene Blood RNA System
  • Single tube for collection, storage, and transport
  • Immediate stabilization of intracellular RNA at collection
  • Transport and store specimens in a range of environments (e.g., room temperature, refrigerated, or frozen)
  • Frozen specimen storage for up to 11 years
  • Standardized preanalytical processing of samples

Product Details

The PAXgene Blood RNA System consists of a blood collection tube (PAXgene Blood RNA Tube) and a nucleic acid purification kit (PAXgene Blood RNA Kit). It is intended for the collection, storage and transport of blood and the stabilization of intracellular RNA in a closed tube, and subsequent isolation and purification of intracellular RNA from whole blood for RT-PCR used in molecular diagnostic testing.

Performance characteristics for the PAXgene Blood RNA System have only been established with FOS* and IL1B** gene transcripts. The user is responsible for establishing appropriate PAXgene Blood RNA System performance characteristics for other target transcripts.

*FOS is the gene symbol for human Fos Proto-Oncogene, AP-1 Transcription Factor Subunit.

**IL1B is the gene symbol for human Interleukin 1 Beta.

Performance

The additive in PAXgene Blood RNA Tubes protects RNA molecules from degradation by RNases and other enzymes and triggers the complete lysis of all cells in the sample to minimize ex vivo changes in gene expression due to gene induction and downregulation. The result is immediate and allows long-term stabilization of RNA in specimens for up to 3 days at room temperature (see figures " RNA stability at 18–25°C: FOS" and " RNA stability at 18–25°C: IL1B") or refrigerated for up to 5 days (see figures " RNA stability at 2–8°C: FOS" and " RNA stability at 2–8°C: IL1B"). Current data shows stabilization of intracellular RNA for up to 11 years at –20°C or –70°C (see Technical Notes).

See figures

Principle

The collection of whole blood is the first step in many molecular assays used to study intracellular RNA. A major challenge in this type of testing is the instability of intracellular RNA, which rapidly degrades within hours after blood collection. Furthermore, certain transcript levels change after blood collection due to ex vivo gene regulation, such as induction and repression. Both RNA degradation and gene regulation can lead to an under- or overestimation of in vivo gene transcript levels.

The PAXgene Blood RNA Tube contains an additive that stabilizes the in vivo gene expression profile by reducing post-collection RNA degradation and minimizing gene induction and repression. When used in conjunction with one of the PAXgene Blood RNA Kits, the PAXgene Blood RNA Tube provides pure RNA for accurate detection and quantification of gene expression.

Procedure

Whole blood is collected directly into the PAXgene Blood RNA Tube, using standard phlebotomy practice and equipment. The RNA in the specimen is immediately stabilized for storage at room temperature, refrigerated, or frozen. RNA can be purified using one of the PAXgene Blood RNA Kits (FDA and CE). The ease of collection, stabilization of RNA at different temperatures, and elimination of additional processing steps, such as centrifugation at the collection facility and storage on ice prior to RNA purification, contributes to standardize the workflow from collection to purification, reducing variability that can be introduced by different operators (see " Reproducibility between users.")

See figures

Applications

When used in conjunction with the PAXgene Blood RNA Kit, the PAXgene Blood RNA Tubes enable purification of intracellular RNA from whole blood for downstream applications in molecular diagnostic testing, such as real-time RT-PCR analysis of specific transcripts.

Supporting data and figures

Specifications

FeaturesSpecifications
Tube size16 x 100 mm
Draw volume2.5 ml
Additive/concentrationAdditive 6.9 ml
Closure type/colorBD Hemogard™ safety closure with transparent shield and red stopper
Label typePaper
Quantity100/box

Resources

Brochures & Guides (2)
For collection, transport, and storage of whole blood and stabilization and purification of intracellular RNA

 

For molecular diagnostic testing

 

Application Notes (2)
Typical total RNA yields from PAXgene Blood RNA Tubes processed with the PAXgene Blood RNA Kit
In situ stability of RNA in blood specimens stored for 11 years (132 months) at –20°C and –70°C in PAXgene Blood RNA Tubes

 

Quick-Start Protocols (1)
How to Collect Blood Using the PAXgene Blood RNA Tube for Molecular Diagnostic Testing
Safety Data Sheets (1)
Certificates of Analysis (1)

FAQ

What are the expected yields of RNA from PAXgene Blood RNA Tubes using the QIAsymphony PAXgene Blood RNA Kit?
RNA yields isolated from 2.5 ml healthy, human, whole blood (4.8 x 106 –1.1 x 107 leukocytes/ml) are specified to ≥3 µg for >95% of the samples processed (see Technical Note for the QIAsymphony PAXgene Blood RNA System ). Since yields are highly donor-dependent, individual yields may vary. Mean yields in the range of 8-10µg per tube or higher. 
FAQ ID - 3475
Where can I find additional information for PreAnalytiX PAXgene products?
You can find additional information relating to the PreAnalytiX PAXgene products on the PreAnalytiX website .
FAQ ID - 3515
Can the PAXgene Blood RNA System be used with animal blood samples?

The PAXgene Blood RNA System was developed for intracellular RNA isolation from human blood samples. QIAGEN offers RNA stabilization and isolation systems for animal blood. Please contact QIAGEN technical service (www.qiagen.com) for more information.

FAQ ID - 3461
What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit?
Typical yields of RNA isolated from 2.5 ml healthy, human, whole blood (4.8 x 106 –1.1 x 107 leukocytes/ml) are ≥3 μg for >95% of the samples processed (Refer to Technical Note) . Since yields are highly donor-dependent, individual yields may vary.
FAQ ID - 3481
What RNA yields are expected with the PAXgene Blood RNA kit?

Typical yields of RNA isolated from 2.5 ml human whole blood are between 4 and 20 µg. However yield is highly donor-dependent, and in some cases higher or lower yields may be achieved. At least 95% of all samples with a WBC count between 4.8 and 11.0 x 106 cells/ml of blood will yield ≥3 µg of total RNA per tube. To prevent low yields due to underfilling of the tube, see the phlebotomy FAQ and the blood collection demonstration video.

FAQ ID - 3464
Why is a blood collection set required to be used with the PAXgene Blood RNA tube?

A blood collection set is required to be used with the PAXgene Blood RNA Tube to eliminate the possibility of patient contact with the reagent in the tube, due to backflow of blood, when used in accordance with the instructions for use.

FAQ ID - 3459
What is the integrity of RNA from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?
Like yield, RNA integrity depends on parameters, such as tissue type, period of cold ischemia, fixation time, processing protocol, age, and storage conditions of the PFPE block. For examples of the integrity of RNA in rat tissues under ideal workflow conditions, see Groelz et al., Exp Mol Pathol. 2013 Feb;94(1). For examples of the integrity of RNA from clinical samples, see Viertler et al., J Mol Diagn. 2012 Sep;14(5)
FAQ ID -3046
How long can the PAXgene Blood RNA tubes be kept frozen at –20°C or –70°C?
Currently we have data for storage at –20°C and –70°C for 96 months (In situ stability of RNA in blood specimens stored for 8 years (96 months) at –20°C and –70°C in PAXgene Blood RNA Tubes).  

Long term archiving studies are ongoing. See technical note: In Situ Stability of RNA in Blood Samples Stored at –20°C and –70°C in PAXgene Blood RNA Tubes.


 
FAQ ID - 3463
Can the PAXgene RNA stabilization solution/blood mixture from the PAXgene Blood RNA Tube be disposed of by adding bleach?

Yes. You may dispose of the waste product from the PAXgene Blood RNA Tube with a 5% sodium hypochlorite bleach solution, in a 1:9 ratio (1 part bleach : 9 parts tube contents).

FAQ ID - 3469
What are possible reasons for blood draws with lower than expected blood volume?

Possible reasons for blood draws with lower than expected blood volume include: 1) The phlebotomist has not waited enough time for the blood to stop flowing into the tube. 2) The tube has "pushed back" from the needle end attached to the holder and the tube is no longer drawing. 3) Discard tube was not used. For further information see the video Collecting Specimens In PAXgene Blood RNA Tubes and Instructions for Use.

FAQ ID - 3462
Is there any chance of the PAXgene Blood RNA tube reagent going back into the patient's arm?

If used according to the instructions for use, there is no possibility of backflow during phlebotomy, and thus no possibility of reagent going into the patient’s arm.

FAQ ID - 3460
Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument?
Yes. Samples have to be incubated at least 2 h at room temperature (18-22°C). Incubation of the PAXgene Blood RNA Tube overnight may increase yields slightly in some cases. Room temperature storage for up to three days has no effect on the RNA profile. During the preparation of the RNA the manual pretreatment consist of a centrifugation and a pellet resuspenion step (see the PAXgene Blood miRNA Handbook ).
FAQ ID - 3479