Transfection Cell Database - Detailed View


Cell Line: NIH/3T3

Cell Line Species/Tissue: Mouse /  
Transfection Reagent: HiPerFect HTS 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
DMEM 
Percent Serum (%):
10% 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
384-well 
Number of Cells:
1,5*10e3 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
10nM 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
0,1µl 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
 
Transfection Efficiency (%):
 
Knockdown Efficiency (%): 86%  
 
Any modifications to the protocol?:
 
Notes:
 
References: