Transfection Cell Database - Detailed View


Cell Line: H4

Cell Line Species/Tissue: Human / Neuroglioma 
Transfection Reagent: RNAiFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
DMEM 
Percent Serum (%):
10% 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
24-well 
Number of Cells:
2x104 
Percent Confluence(%):
70-80% 
Amount of Nucleic Acid (µg):
1µg 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
6µl 
Complex Incubation on Cells (hrs):
3h 
Analysis Performed Post-Transfection (hrs):
72h 
Transfection Efficiency (%):
Not determined 
Knockdown Efficiency (%): Worked  
 
Any modifications to the protocol?:
No  
Notes:
 
References: