Transfection Cell Database - Detailed View


Cell Line: Swiss 3T3

Cell Line Species/Tissue: Mouse / Fibroblast 
Transfection Reagent: PolyFect 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10 % 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
6-well plate 
Number of Cells:
 
Percent Confluence(%):
60-70 % 
Amount of Nucleic Acid (µg):
1,5 µg 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
10 µl 
Complex Incubation on Cells (hrs):
4-6 h 
Analysis Performed Post-Transfection (hrs):
48 h 
Transfection Efficiency (%):
20-40 % 
 
Any modifications to the protocol?:
 
Notes:
 
References: