AllPrep Bacterial/Fungal DNA/RNA/Protein Kits

For extraction of nucleic acids from bacterial and fungal cell cultures

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AllPrep Fungal DNA/RNA/Protein Kit (50)

Cat. No. / ID: 47154

For the extraction of protein and nucleic acids from fungal cell cultures

Sample type

Bacterial cell cultures

Fungal cell cultures

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AllPrep Bacterial/Fungal DNA/RNA/Protein Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Efficient solubilization of total nucleic acids and cellular proteins from bacterial and fungal samples
Sequential DNA, RNA and protein extraction in less than 40 minutes without precipitation
Ready-to-use, high-quality nucleic acids and proteins for downstream applications

Product Details

Easy-to-use AllPrep Bacterial/Fungal DNA/RNA/Protein Kits isolate total nucleic acids and cellular proteins from Gram (+/-) bacterial cultures and fungal cell cultures. The bead-beating method and spin column technology effectively extract nucleic acids and proteins in a streamlined process. DNA and RNA are ready for qPCR, RT-PCR and next-generation sequencing, while protein is ready for 1D SDS-PAGE and mass spectrometry following in-gel trypsin digestion. Some applications, including 2D SDS-PAGE and solution phase proteolytic digestion for mass spectrometry, may require an additional detergent removal step following protein elution.

Procedure

Cultured bacterial and fungal cells are first lysed using glass bead tubes, and nucleic acids and proteins are solubilized and mixed with a binding solution. DNA binds to the first spin column and the flow-through is combined with a solution to bind the total RNA on a second spin column. The final flow-through captures protein. Each of the individual spin columns is then washed and the immobilized analyte is eluted.

Comparison of Allprep Bacterial/Fungal DNA/RNA/Protein Kits
Features	Allprep Bacterial DNA/RNA/Protein Kit	Allprep Fungal DNA/RNA/Protein Kit
Bead size	0.1 mm glass	0.5 mm glass
Format	Silica Spin Filter	Silica Spin Filter
Processing	Chemical lysis and/or bead beating	Chemical lysis and/or bead beating
Sample size	1.8 ml of microbial culture QIAGEN recommends using no more than 1x10⁹ bacterial cells per sample	1.8 ml of fungal culture
Sample types	Cultured Gram-negative and Gram-positive bacteria	Cultured fungi
Storage temperature	Store spin filters at 4°C. All other kit reagents and components can be stored at room temperature (15–30°C).	Store spin filters at 4°C. All other kit reagents and components can be stored at room temperature (15–30°C).
Throughput	1–24 samples	1–24 samples
Time per run or per prep	36 minutes	36 minutes