QIAamp DSP Viral RNA Mini Kit

For purification of viral RNA from cell-free body fluids

Features

Rapid isolation of high-quality, ready-to use RNA
No organic extraction or alcohol precipitation
Consistent, high yields
Removal of contaminants and inhibitors

Product Details

The QIAamp DSP Viral RNA Mini Kit provides silica-based viral RNA purification and sample concentration for those samples with low viral titers. The kit is designed for labs that process viral RNA for in vitro diagnostic use. The procedure can be fully automated on the QIAcube.

Performance

The QIAamp DSP Viral RNA Mini Kit includes QIAamp Mini spin columns, which can be used in a microcentrifuge, automated on the QIAcube, or processed on vacuum manifolds (i.e., the QIAvac 24 Plus with VacConnectors and VacValves). The sample input volume is 140 µl. The QIAamp DSP Viral RNA mini Kit purifies viral RNA for downstream applications.

Principle

No phenol–chloroform extraction is required. Viral RNA binds specifically to the QIAamp silica membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are removed in two efficient wash steps, leaving viral RNA to be eluted in either water or elution buffer provided with the kit.

Procedure

Viral RNA binds selectively to the QIAamp silica membrane, and purified viral RNA is eluted in either water or elution buffer provided with the kit (see ""). If automating the QIAamp DSP Viral RNA Mini Kit on the QIAcube instrument, the instrument may process fewer than 50 samples due to dead volumes, evaporation, and additional reagent consumption by automated pipetting. QIAGEN only guarantees 50 sample preps with manual use of the QIAamp DSP Viral RNA Mini Kit.

See figures

QIAamp DSP Viral RNA Mini Kit procedure.

Applications

The QIAamp DSP Viral RNA Mini Kit simplifies purification of viral RNA from cell-free body fluids, plasma (treated with anticoagulants other than heparin) or serum with fast, spin-column, or vacuum procedures.

Supporting data and figures

QIAamp DSP Viral RNA Mini Kit procedure.

Resources

FAQ

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

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