3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits?

It is not recommended to use more than 10ng for the PCR reaction in the therascreen BRAF, therascreen EGFR, therascreen KRAS,  therascreen NRAS Pyro, and therascreen UGT1A1 Kits.  Too much DNA can sometimes lead to inhibition problems in the PCR reaction.