Can I adjust the ROX concentration in the QuantiFast master mix?

The master mix in QuantiFast SYBR Green Kits contains an optimized concentration of ROX dye that works well with all cyclers.

QuantiFast Probe PCR Kits are available in two formats:

• the QuantiFast Probe PCR Kit with master mix containing ROX dye
• the QuantiFast Probe PCR +ROX Vial Kit with master mix not containing ROX dye, and a separate vial of ROX dye

We recommend using the latter with the Applied Biosystems 7500 Fast System. Use the ROX concentration indicated in the QuantiFast Probe PCR Kits handbook.