Can I use the SureSilencing shRNA Plasmids with primary cells or macrophages or for injection into live animals?

No, we do not recommend using the SureSilencing shRNA Plasmids with primary cells or macrophages or other cell lines that tend to be difficult to transfect with expression plasmids by traditional methods. Similarly, we do not recommend using the SureSilencing shRNA Plasmids for RNA interference in live animals. The SureSilencing shRNA Plasmids are meant for in vitro use only. The delivery of any method of RNA interference into live animals to specific target tissues or organs is a difficult procedure for which no manufacturer has a viable solution.