What is the miRNA yield from the miRNeasy Serum/Plasma Kit?

Yields of total RNA achieved with the miRNeasy Serum/Plasma Kit vary strongly between samples from different individuals. However, yield of RNA from 50-200 µl human plasma and serum is not quantifiable with photometric methods like NanoDrop; they are usually too low for quantification by OD measurement.

You may use more sensitive colorimetric or fluorimetric assays for quantification, i.e. RiboGreen assays.

Use of miRNeasy Serum/Plasma Spike-In Control (cat. no. 219610) and corresponding Ce_miR-39_1 miScript Primer Assay (cat.no. MS00019789) is recommended to monitor miRNA purification and amplification.