QuickLyse Miniprep Kit

For fast purification of up to 15 µg sequencing-grade plasmid

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QuickLyse Miniprep Kit (250)

Cat. No. / ID: 27406

250 QuickLyse Spin Columns, Reagents, Buffers, Lysis and Collection Tubes (2 ml)

The QuickLyse Miniprep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Rapid one-step lysis procedure
Reduced protocol steps and hands-on time
Up to 15 µg DNA from 1.5 culture volumes obtained
Ready-to-use plasmid DNA in just 9 minutes

Product Details

The QuickLyse Miniprep Kit provides a rapid spin-column-based method for isolating sequencing-grade plasmid DNA. Bacterial cell resuspension, lysis, and DNA binding are achieved in a single buffer, enabling fast DNA purification with minimal hands-on time.

Performance

The QIAGEN QuickLyse Miniprep Kit uses one-step lysis technology for ultrafast preparation of up to 15 µg plasmid DNA. The quality of the DNA ensures robust performance in applications, such as sequencing, restriction analyses, and cloning (see figure " Reproducible yields").

The QuickLyse Miniprep Kit yields sufficient plasmid DNA for any downstream application, including sequencing (see figure " Long read-lengths in automated sequencing"). Up to 15 µg of high-copy plasmid DNA can be obtained from 1.5 ml of bacterial culture (see figure " High yields of high-quality plasmid DNA"). Isolated plasmid DNA has OD₂₆₀_/280 ratios of 1.7–1.9, indicating that the quality and purity is suitable for all sensitive applications. Sequencing read lengths of over 700 bases are easily achieved.

See figures

Reproducible yields.

Long read-lengths in automated sequencing.

High yields of high-quality plasmid DNA.

Principle

Unlike most protocols that use a 3-step lysis procedure, the QuickLyse Miniprep Kit combines enzymatic and osmotic processes to lyse bacterial cells in a single, 3-minute step. In addition, QuickLyse technology uses fewer buffers, simplifying handling and saving time: 24 plasmid DNA minipreps can be prepared in less than 22 minutes.

Procedure

Bacterial cells are resuspended and lysed and DNA is bound in a single buffer, enabling fast DNA purification with minimal hands-on time. After a 3-minute lysis step, the clear lysate is applied directly to a QuickLyse Spin Column. Plasmid DNA binds to the column membrane and is eluted after a single wash step (see flowchart ""). The isolated DNA can be used directly in standard applications, including automated sequencing, PCR, restriction analysis, and cloning.

See figures

QuickLyse procedure.

Applications

The QuickLyse Miniprep Kit yields sufficient plasmid DNA for any downstream application, including automated sequencing.

Supporting data and figures

High yields of high-quality plasmid DNA.

Typical DNA yield and purity from 1.5 ml of bacterial culture (LB media) for different cells and vectors.

Specifications

Features	Specifications
Applications	Automated sequencing, PCR, restriction analysis, cloning etc.
Time per run or prep per run	9 min
Culture volume/starting material	1.5 ml culture volume
Plasmid type	High-copy, cosmid DNA
Elution volume	50 µl
Samples per run (throughput)	1–24 samples per run
Technology	QuickLyse technology
Yield	up to 15 µg
Processing	Manual (centrifugation)

Resources

For purification of sequencing grade plasmid DNA

FAQ

The QuickLyse Miniprep Kit can be used for plasmids up to 9 kb in size. It is not recommended for Large-Construct purifications.

Please find an overview of additional kit options for small-scale Plasmid Purification on the QIAGEN website, or use our ProductFinder to select the right kit for your needs.

FAQ ID -1752

No, this is not possible since the columns do not fit properly on the QIAVac 24 Plus manifold and the vacuum is not strong enough.

Normally, 1.5 ml bacterial culture is used with the QuickLyse Miniprep Kit. Only if the OD600 is < 2.0, up to 3 ml culture may be used. Growth in LB medium is strongly recommended. Growth in nutrient-rich media, such as 2xYT, is not recommended.

FAQ ID -1357

When using the QuickLyse Miniprep Kit, the addition of 125 µl isopropanol to the lysate, prior to transferring it to the QuickLyse Spin Column, may increase plasmid DNA yield in some cases.

FAQ ID -1354

No. Vortexing for 30 seconds as described in the QuickLyse Miniprep Handbook is absolutely critical to resuspend and lyse the bacterial cells. Failure to follow this step will result in greatly reduced DNA yields.

FAQ ID -1352

Elution Buffer QLE of the QuickLyse Miniprep Kit contains 10 mM Tris-Cl and 0.1 mM EDTA (pH 8.5). Due to the very low concentration of EDTA, enzymatic downstream reactions such as PCR and cycle sequencing are not inhibited.

FAQ ID -1356

Yes, nuclease-free water may be used for the elution of plasmid DNA purified with the QuickLyse Miniprep Kit. However, for higher DNA stability and optimal yields, it is recommend to elute using the provided elution buffer (Buffer QLE).

FAQ ID -1355