How stable is Phoenix Hot Start Taq when incubated in a PCR reaction mix at room temperature?
Phoenix Hot Start Taq is stable at room temperature for at least 72 hours when incubated in a PCR reaction mix containing 1X of the supplied, optimized Phoenix Hot Start Taq reaction buffer (standard or GC). Similar testing on a smaller number of amplicons demonstrated functional stability by endpoint PCR following 20 days room temperature incubation.