What is the cell number range one can use with the FastLane Cell cDNA Kit for real-time RT-PCR?
The FastLane Cell cDNA Kit enables accurate detection over a wide linear range from 1 x 105 cells down to one cell in real-time RT-PCR. Data is shown on our website in the figure 'Linear Detection in Real-Time RT-PCR Down to One Cell'.
For optimized FastLane Buffer volumes (FCW and FCP) per cell number seeded, please see Appendix C of the FastLane Cell cDNA Handbook: 'Seeding and Processing Cells for Different Plate Formats'.