How long can blood samples be stored before sample preparation using the EZ1 DSP DNA Blood system?
For short-term storage (up to 7 days), collect blood in tubes containing EDTA as an anticoagulant and store the tubes at 2–8°C. However, for applications requiring maximum fragment size, such as southern blotting, we recommend storage at 2–8°C for up to 3 days only, as low levels of DNA degradation will occur after this time.
For long-term storage, collect blood in tubes containing a standard anticoagulant (preferably EDTA, if high-molecular-weight DNA is required) and store the tubes at −20°C for a maximum of 4 weeks. Longer storage may be possible depending on the downstream application but needs to be validated by the user.
Yield and quality of the purified DNA may depend on storage conditions of the blood. Fresh blood samples may yield better results. Do not re-freeze blood samples more than 2 times as this may result in decreasing DNA yield.
For long-term storage, collect blood in tubes containing a standard anticoagulant (preferably EDTA, if high-molecular-weight DNA is required) and store the tubes at −20°C for a maximum of 4 weeks. Longer storage may be possible depending on the downstream application but needs to be validated by the user.
Yield and quality of the purified DNA may depend on storage conditions of the blood. Fresh blood samples may yield better results. Do not re-freeze blood samples more than 2 times as this may result in decreasing DNA yield.