What is the effect of using amounts of input material per sample that fall outside the recommended range?

Using input amounts outside the range of 5 x 10³ and 2.5 x 10⁶ human or mouse cells (or the equivalent of 30 ng–15 µg DNA) per sample risks affecting the quality of the resulting NGS library, such that it is likely to have higher levels of inward-facing (FR) sequence read bias, unligated ends, and read pairs containing contiguous, religated restriction fragments.