What is the genotype of the EZ Competent Cells?
Genotype of QIAGEN EZ Competent Cells supplied in the QIAGEN PCR Cloning plus Kit:
| Genotype | Description/phenotype |
| F' | Presence of the low-copy-number F plasmid [F'::Tn10(Tcr) proA+ B+ lacIq Z delta-M15] |
| Tn10 (Tcr) | Transposon conferring tetracyclin resistance |
| lacIq | Overproduction of the lac operon repressor, which controls expression from Plac |
| lacZ delta-M15 | Expression of N-terminally deleted beta-galactosidase.This protein and LacZ alpha-peptide (encoded by the pDrive Cloning Vector) provide alpha-galactosidase activity. Cells transformed by pDrive Cloning Vector which does not contain a PCR product will express LacZ alpha-peptide, and will form blue colonies when grown in the presence of X-gal/IPTG. In contrast, cells transformed by pDrive Cloning Vector which does contain a PCR product will not express LacZ alpha-peptide, and will form white colonies. |
| recA1 | Abolished homologous recombination |
| end A1 | Abolished nonspecific endonuclease I activity, and hence improved DNA quality in plasmid preparations |
| hsdR17 | This mutation prevents restriction, but not protective methylation, of unmethylated DNA or DNA containing foreign methylation patterns in E. coli cells. |
| lac | Inability to utilize lactose |
| glnV44 | Supressor of amber (UAG)mutation; required for growth of some phage vectors. Formerly called supE |
| thi-1 | Requires thiamin (vitamin B1) for growth in minimal medium |
| gyrA96 | Mutation in DNA gyrase; resistance to naladixic acid |
| relA1 | Relaxed mutation, permits RNA synthesis in the absence of protein synthesis |