How can I check the quality or size of my extracted circulating cell-free DNA?

Generation of size-distribution profiles for circulating cell-free DNA (ccfDNA) from eluates can be used to check the quality of fragmented ccfDNA and potential genomic DNA background. However, due to the limited sensitivity of these assays, they are not recommended for quantification of ccfDNA. Samples with low ccfDNA eluate concentration and, thus, low ccfDNA yields might exhibit no peak at approximately 165 bp in size-distribution profile results.