How long should I wait after transfection before enriching my transfected cells, validating knock down, and looking for phenotypic changes?

We recommend starting your FACS-based enrichment or your neomycin (or puromycin) selection 24 to 48 hours after transfection with the SureSilencing shRNA Plasmids. This amount of time is typically sufficient to begin detecting expression of the selectable markers (GFP, puromycin-resistance, neomycin-resistance), and observe the onset of the shRNA-mediated knock down of gene expression.