What programs does your Sequencing Service use to ensure accurate base calling, Sequence Quality, and Alignment?

QIAGEN's Sequencing Services use Phrap and Phred programs for sequence assembly and base calling, respectively. The Staden Package is used for contig assembly, support for confidence scoring, heterozygote detection, and comparative sequencing. Quality clipping of individual sequence reads is performed with phred = 20 values, which represents a sequence accuracy of 99% for single sequence reads.

While the base calling and sequence trimming statistics are run routinely for all sequences to ensure most accurate sequence reads, additional bioinformatic analysis is available upon request tailored to your specific needs. Please submit the Quote and Information Request Form to receive details for your specific project.