How much detection reagent do you generally recommend for LiquiChip Assays?

For antibody-mediated direct detection, use of 10–50 ng labeled antibody per assay point is recommended. For indirect detection, 10 ng primary antibody and 50–200 ng labeled secondary antibody per assay point deliver optimal results.

The LiquiChip Applications Handbook contains protocols for immunoassay and subsequent detection using both direct and indirect methods.