3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?

This allows the genome-wide design of very short assays which are particularly well suited for the detection of highly fragmented transcripts (FFPE samples). In addition, it allows the easy use of gDNA as a positive control for the assay performance.  The gDNA should be eliminated during the RNA isolation or RT-PCR amplification steps.