What are the major differences between QIAseq Targeted RNA Panel TCR kit and previous QIAseq Immune Repertoire RNA library kit?
The new QIAseq Targeted RNA Panel has several improvements. First, it was optimized to start with lower amounts of sample. The UMI used in this kit is longer, so that it can accommodate more T-cell clonotypes without risk of UMI collision. This is necessary when working with samples that have high amounts of T-cells. Lastly, an improvement in library design allows the kit to handle low diversity samples, but still obtain high sequencing Quality scores.