QIAsymphony DSP DNA Kits

For automated purification of DNA from 1–96 samples

Products

QIAsymphony DSP DNA Kits are intended for in-vitro diagnostic use.
Image

QIAsymphony DSP DNA Midi Kit (96)

Cat no. / ID.   937255

For 96 preps of 1000 µl each or 144 preps of 400 µl each: Includes 2 reagent cartridges and enzyme racks and accessories.
$1,375.00
Image

QIAsymphony DSP DNA Mini Kit (192)

Cat no. / ID.   937236

For 192 preps of 200 µl each: Includes 2 reagent cartridges and enzyme racks and accessories.
$1,420.00
The QIAsymphony DSP DNA Mini Kit (192) is intended for in vitro diagnostic use.

Features

  • Purification of DNA from a broad range of sample types
  • Novel, prefilled reagent cartridges for ease of use
  • High reproducibility through standardized processing
  • Bar codes enable tracking of samples and reagents
  • For in vitro diagnostic use

Product Details

QIAsymphony DSP DNA Kits, in combination with the QIAsymphony SP, enable automated total DNA purification from human whole blood, buffy coat, tissues and FFPE tissues, as well as viral DNA from human whole blood for in vitro diagnostic use. Kits are available in mini and midi formats for sample volumes ranging from 200 µl to 1000 µl. For tissue and FFPE tissue protocols, manual sample pretreatment is required. Optimized protocols provide high yields of pure DNA, and flexible elution volumes allow DNA concentration to be easily optimized for each downstream application. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

Principle

QIAsymphony DSP DNA Kits can be used to purify total DNA from human whole blood, buffy coat, tissues and FFPE tissues, as well as viral DNA from human whole blood.

QIAsymphony DSP DNA Kits combine the speed and efficiency of silica-based purification of DNA with the convenient handling of magnetic particles (see figure " Schematic of the QIAsymphony SP principle"). Innovative, ready-to-run reagent cartridges are prefilled with all reagents required for the purification procedure and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure " Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves you valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.

Procedure

The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute (see figure "QIAsymphony DSP DNA procedure"). Optimized protocols provide high quality nucleic acids with efficient removal of proteins, nucleases, and other impurities. Nucleic acids purified with QIAsymphony DSP DNA Kits are ready for direct use in downstream applications such as amplification. The user can choose between different elution volumes.

Supporting data and figures

Resources

Kit Handbooks (2)
QIAsymphony DSP DNA Kit Instructions for Use (Handbook)
PDF (939.6KB)
Download
HB-0977-1069185_HB_QS_DSP_DNA_Kit_0815_WW
PDF (647.9KB)
Download
Labware File Documents (1)
(BG) Labware List — QIAsymphony DSP DNA Kits (SOW-516-7)
PDF (282.9KB)
Download
For use with software version 4.0 or higher                    
Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Protocols (15)
QIAsymphony DSP DNA Midi Kit Instructions for Use (Protocol Sheet)
PDF (223KB)
Download
(BG) QIAsymphony SP Protocol Sheet DNA_Blood_1000_V7_DSP protocol
PDF (145.1KB)
Download
For use with software version 4.0 or higher
QIAsymphony DSP DNA Midi Kit Instructions for Use (Protocol Sheet)
PDF (215.7KB)
Download
(BG) QIAsymphony SP Protocol Sheet DNA_Blood_200_V7_DSP protocol
PDF (145.7KB)
Download
For use with software version 4.0 or higher
(BG) QIAsymphony SP Protocol Sheet Tissue_LC_200_V7_DSP and Tissue_HC_200_V7_DSP protocol
PDF (184.7KB)
Download
For use with software version 4.0 or higher
(BG) QIAsymphony SP Protocol Sheet DNA_Blood_400_V6_DSP protocol
PDF (146.6KB)
Download
For use with software version 4.0 or higher
(BG) QIAsymphony SP Protocol Sheet DNA_Buffy_Coat_400_V6_DSP protocol
PDF (147.8KB)
Download
For use with software version 4.0 or higher
QIAsymphony DSP DNA Mini Kit Instructions for Use (Protocol Sheet)
PDF (206.9KB)
Download
(BG) QIAsymphony SP Protocol Sheet DNA_Buffy_Coat_200_V7 DSP protocol
PDF (147.4KB)
Download
For use with software version 4.0 or higher
(BG) QIAsymphony SP Protocol Sheet VirusBlood200_V5_DSP protocol
PDF (157.7KB)
Download
For use with software version 4.0 or higher
QIAsymphony DSP DNA Mini Kit Instructions for Use (Protocol Sheet)
PDF (298.8KB)
Download
QIAsymphony DSP DNA Mini Kit Instructions for Use (Protocol Sheet)
PDF (207.1KB)
Download
QIAsymphony DSP DNA Mini Kit Instructions for Use (Protocol Sheet)
PDF (259.6KB)
Download
QIAsymphony DSP DNA Mini Kit Instructions for Use (Protocol Sheet)
PDF (223KB)
Download
Tissue_LC_200_V7_DSP and Tissue_HC_200_V7_DSP; Version 2
Technical Information (1)
Important Note: Release of QIAsymphony Labware Package SOW-516-9
PDF (396.9KB)
Download
Certificates of Analysis (1)