Can the Qproteome Plasma Membrane Protein Kit be used with tissue samples?
No, unfortunately it is not possible to use tissue samples with the Qproteome Plasma Membrane Protein Kit. Tissue samples require a completely different lysis procedure.
The Qproteome Plasma Membrane procedure starts with gently lysing only plasma membranes of cultured adherent cells. During subsequent steps, pure plasma membrane vesicles containing plasma membrane proteins are formed. This procedure cannot be performed with tissue samples as a starting material.