Can RNAi experiments be performed in Drosophila?
Yes. Below are a few selected references you can review:
- Elbashir SM, Martinez J, Patkaniowska A, Lendeckel W, Tuschl T. "Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate." EMBO J. 2001, 20 (23): 6877-88
- Zamore PD, Tuschl T, Sharp PA, Bartel DP. "RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals." Cell. 2000, 101 (1): 25-33
- Williams RW, Rubin GM. "ARGONAUTE1 is required for efficient RNA interference in Drosophila embryos." Proc Natl Acad Sci U S A. 2002, 99 (10): 6889-94