PyroMark Systems for Verification and Validation of NGS and Other Genomewide Screening Results
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In this webinar, Gerald Schock will discuss the use of Pyrosequencing technology for verification and validation of NGS and other genomewide screening results. NGS and hybridization arrays unlock genomewide information and identify targets whose biological relevance must be experimentally demonstrated. Systematic verification and validation of such data typically involve increased sample numbers, so cost-effective techniques for analyzing several hundreds of samples with high resolution and sensitivity are essential.
QIAGEN’s PyroMark platforms are ideally suited to analyze short- to medium-length sequences quantitatively with high accuracy. Compared to other widely used validation technologies, Pyrosequencing delivers both sequence information as well as highly sensitive quantification results. This technology has already been used in several studies to validate somatic mutation or methylation data.
The presentation will focus on the following topics
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In this webinar, Gerald Schock will discuss the use of Pyrosequencing technology for verification and validation of NGS and other genomewide screening results. NGS and hybridization arrays unlock genomewide information and identify targets whose biological relevance must be experimentally demonstrated. Systematic verification and validation of such data typically involve increased sample numbers, so cost-effective techniques for analyzing several hundreds of samples with high resolution and sensitivity are essential.
QIAGEN’s PyroMark platforms are ideally suited to analyze short- to medium-length sequences quantitatively with high accuracy. Compared to other widely used validation technologies, Pyrosequencing delivers both sequence information as well as highly sensitive quantification results. This technology has already been used in several studies to validate somatic mutation or methylation data.
The presentation will focus on the following topics
Pyrosequencing technology and workflow
Comparison to other validation technologies such as Sanger sequencing and real-time PCR
Latest advances in instruments, chemistry, and software
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