| Cell Line Species/Tissue: |
mouse
/
embryo fibroblast |
| Transfection Reagent: |
Attractene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
10% |
Reporter System:
|
ß-Galactosidase |
Plasmid Purification Method:
|
|
Plate Format:
|
96-well |
Number of Cells:
|
2x 10^4 (seeded one day before) |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0,2 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
2 µl |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
80U |
| |
| Any modifications to the protocol?:
|
| Yes, final volume of diluted DNA was 30 µl instead of 50 µl |
| Notes: |
| |
| References: |
| |