| Cell Line Species/Tissue: |
human
/
kidney epithelial |
| Transfection Reagent: |
PolyFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM containing Glutamax, 4,5g Glucose, 10%FCS, 1%NEAA |
Percent Serum (%):
|
10 |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
|
Plate Format:
|
75cm3 |
Number of Cells:
|
|
Percent Confluence(%):
|
60-80 |
Amount of Nucleic Acid (µg):
|
12 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
115 |
Complex Incubation on Cells (hrs):
|
24/48 |
Analysis Performed Post-Transfection (hrs):
|
24/48 |
Transfection Efficiency (%):
|
90% |
| |
| Any modifications to the protocol?:
|
| Wash cells once with 15ml PBS during complex formation Transfection in a T75 flask |
| Notes: |
| |
| References: |
| |