| Cell Line Species/Tissue: |
mouse
/
fibroblasts (NIH/3T3 derived) |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DME |
Percent Serum (%):
|
8 |
Reporter System:
|
GFP, pGreenLantern-1 (LTI) |
Plasmid Purification Method:
|
QIAGEN Plasmid Kits |
Plate Format:
|
24-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
40-80 |
Amount of Nucleic Acid (µg):
|
1 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
5 |
Complex Incubation on Cells (hrs):
|
2-5 |
Analysis Performed Post-Transfection (hrs):
|
24-36 |
Transfection Efficiency (%):
|
14-28% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| Albritton L.M. (1997) Efficient transfection of fibroblast and epithelial cells with SuperFect Transfection Reagent. QIAGEN News 1997 No. 2, 4 |