| Cell Line Species/Tissue: |
rabbit
/
gastric parietal |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DME F12 |
Percent Serum (%):
|
0 |
Reporter System:
|
GFP, pEGFP-N2 (ClonTech) |
Plasmid Purification Method:
|
EndoFree Plasmid Kit |
Plate Format:
|
35-mm |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.4 |
Amount of Enhancer (µl):
|
3.2 |
Amount of Reagent (µl):
|
4 |
Complex Incubation on Cells (hrs):
|
24 |
Analysis Performed Post-Transfection (hrs):
|
24 |
Transfection Efficiency (%):
|
21% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| J.A. and Chew, C.S. (1998) Combined effect of transfection reagent and plasmid DNA quality on transfection of primary rabbit gastric cells. QIAGEN News 1998 No. 5, 1 |