| Cell Line Species/Tissue: |
rat
/
myoblast, skeletal muscle |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
MEM, 10% FCS |
Percent Serum (%):
|
10 |
Reporter System:
|
GFP, pEGFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.4 |
Amount of Enhancer (µl):
|
3.2 |
Amount of Reagent (µl):
|
5 |
Complex Incubation on Cells (hrs):
|
10 |
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
20% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Approximately same efficiency with SuperFect. Effectene more gentle. Electroporation gave 5%. |
| References: |
| |