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3225 - How pure should my protein be for the NexTal CubicPhase kits?
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3226 - How pure should the chloroform used with the RNeasy Plus Universal Mini Kit be?
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3227 - In which solution can I keep my protein with the NexTal Cubic Phase kits?
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3228 - Is it also possible to isolate miRNA with the AllPrep DNA/RNA FFPE Kit?
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3229 - Is it necessary to perform calibration steps for the use of the MAX dye in duplex RT-PCR experiments on different cyclers?
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3231 - Is there a size limit to the protein I can crystallize with the NexTal Cubic Phase products?
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3232 - Is the RNeasy Plus Universal Mini Kit suited for adipose, brain, and other fatty tissues?
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3233 - Is the yield and quality of RNA and DNA purified with the AllPrep DNA/RNA FFPE Kit comparable to yield and quality of nucleic acids purified separately with other methods.
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3236 - I use cultured cells as starting material. Can I also use the RNeasy Plus Universal Kit?
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3237 - I would like to order the QuantiFast Probe Assays without master mix. Is this possible?
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3238 - What are important points regarding the genomic DNA wipeout buffer in the QuantiFast Probe RT-PCR Plus Kit?
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3239 - What are the differences between the existing QuantiFast Probe RT-PCR Kit and the new Plus Kit?
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3240 - What are the main differences between the qBiomarker PCR Arrays and the RT2 Profiler PCR Arrays?
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3241 - What are the recommended storage conditions of the QuantiFast Probe RT-PCR Plus Kit components?
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3243 - What does the Investigator Nonaplex ESS Kit contain?
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3244 -What is the average length of a QuantiFast Probe Assay?
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3245 - What is the concentration of the primers and the probe in a reaction using the QuantiFast Probe Assays?
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3246 - What is the correct matrix to use with Investigator ESSplex SE Plus Kit?
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3247 - What is the correct matrix to use with the Investigator Nonaplex ESS Kit?
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3248 - What is the difference between sponge, cubic and lamellar phase?
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3249 - What is the optimal DNA volume and concentration for the Investigator Nonaplex ESS Kit?
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3252 - What should be the concentration of my protein with the NexTal Cubic Phase products?
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3253 - What species are QuantiTect Probe Assays offered for?
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3254 - When using the Nextal Cubic Phase products how do I know in which phase the sample is? What will I see?
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3255 - When using the NexTal Cubic Phase products, should I work with diluted or undiluted screens?
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3256 - Which crystallization solutions are compatible with lipidic cubic phase crystallization?
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3257 - Which deparaffinization method can I use with the AllPrep DNA/RNA FFPE Kit?
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3258 - Which filter set and settings should be used with the Investigator Nonaplex ESS Kit?
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3259 - Which probe labels are available for the QuantiFast Probe Assays?
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3260 - Which purification kits are recommended for the Pyro kits?
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3261 - Which purification kits are recommended for the therascreen Pyro kits?
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3262 - Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit?
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3263 - Why are samples centrifuged at 4°C after the addition of chloroform when using the RNeasy Plus Universal Mini Kit?
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3264 - Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
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3265 - Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
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3266 - Why are two different exon 2 forward sequencing primers used for the SBTexcellerator HLA-DRB1 Kit?
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3270 - Are the primer sets different for the Investigator Nonaplex ESS Kit and ESSplex SE where the loci are the same?
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3272 - Are the QuantiFast Probe Assays wet-lab validated?
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3273 - At what temperature can I perform the experiment with the NexTal Cubic Phase kits?
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3274 - Can I do singleplex PCR with the QuantiFast Probe Assays?
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3275 - Can I use more than 10ng DNA for the PCR reaction for my therascreen Pyro Kits?
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3277 - Can I use the RNeasy Plus Universal Mini Kit for all types of tissue?
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3283-Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
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3284-For which applications can the RNA isolated with the RNeasy Plus Universal Kit also be used?
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3285-How are the genes in each iPSC qBiomarker PCR Array selected?
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3286-How can I adjust the ROX concentration in the QuantiFast Probe RT-PCR Plus master mix?
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3288-How does the gDNA eliminator solution of the RNeasy Plus Universal Kit work?
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3289-How does the QuantiFast Probe RT-PCR Plus Kit eliminate genomic DNA contamination?
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3290-How do I analyze the data for the particular iPSC qBiomarker Arrays I have ordered?
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3291-How do I harvest the crystals from meso phase? Are special tools needed with the NexTal Cubic Phase products?
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3292-How do I start using the NeXtal CubicPhase kits?
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3294-How long can I store my purified DNA/RNA isolated with the AllPrep DNA/RNA FFPE kit?
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3295-What is the difference between SBTexcellerator HLA Core, Extended and Full Kits?
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3296-Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit?
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3297-Why does the PCR for HLA-DRB4 result in 2 amplicons of different size?
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3298-Will the sequence of the QuantiFast Probe Assays be provided?
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3299-Can Buffer ER from the Endofree Plasmid kits be used instead of the Buffer ETR in QIAGEN Plasmid Plus kits?
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3300 - Can isopropanol or methanol be used in place of ethanol in RNeasy kits?
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3301 - Do you have any information or guidelines regarding the choice of reference genes for real-time PCR?
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3302 - How long can I store my purified RNA?
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3304 - What is the maximum DNA input volume that can be used in the Investigator ESSplex SE Plus Kit?
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3305 - What is the minimal amount of starting material required for using the SBTexcellerator HLA Kits?
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3306 - What is the optimal sample DNA concentration for the Investigator ESSplex SE Plus Kit?
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3308 - Where can I find the template files for the Investigator ESSplex SE Plus Kit?
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3309 - Which filter set should be used with Investigator ESSplex SE Plus Kit?
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3310 - Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit?
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3311 - Can QuickLyse miniprep kits be used with QIAVac 24 Plus?
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3312 - For DirectPrep 96 miniprep, is it possible to use a half plate and save the other half for later prep?
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3313 - What is the endotoxin level for the QIAGEN Plasmid Plus kits?
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3314-What is the concentration of RNase A sold separately?
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3315-What is the concentration of the reconstituted QuantiTect Primer Assay (200)?
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3316-What is the concentration of the reconstituted QuantiFast Probe Assay?
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3317 - Do you have a protocol for isolation of genomic DNA from cell cultures using the QIAamp cador Pathogen Mini Kit?
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3318 - What guidelines should I follow in designing primers for use with the EpiTect MSP Kit?
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3318 - What is the preferred amplicon size for performing PCR on DNA amplified with the EpiTect Whole Bisulfitome Kit?
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3320 - What is the sensitivity of the Type-it HRM PCR Kit?
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3321 - What is the purpose of the C. elegans miR-39 spike-in control in the miRNeasy Serum/Plasma kit?
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3322 - What is the concentration of the reconstituted miScript Primer Assay (100) and miScript Precursor Assay (100) tubes?
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3323 - Do you have a protocol for the AllPrep DNA/RNA/Protein Mini Kit on the QIAcube?
FAQ ID - 3323
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3324 - Do you have a protocol for the QIAprep Spin M13 Kit on the QIAcube?
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3325 - What are the catalog numbers for the packing materials for the QIAcube?
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3326 - Which QIAcube standard protocol might be suitable to extract RNA from saliva or from a buccal cell pellet?
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3327 - How often should the o-ring for the pipettor tip-adapter be changed on the QIAcube?
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3328 - Can the Sample Tubes CB and the Sample Tubes RB be used interchangeably?
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3329 - When using REPLI-g, if the starting material is a single sorted cell, could mitochondrial DNA (mtDNA) be amplified?
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3330 - Is there any contamination from E. coli genomic DNA in the polymerase provided with the Repli-G Single Cell Kit?
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3331 - Should the REPLI-g amplified mtDNA be cleaned up before sequencing?
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3332 - Is there a method that can distinguish between non-amplified DNA and REPLI-g amplified DNA?
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3333 - When using REPLI-g, what is the purpose of the DTT added to the denaturation buffer in the cells/blood protocol?
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3335 - Can I use the DNA directly from enzymatic reaction for Epitect Bisulfite treatment with the Epitect Bisulfite kit (cat. no. 59104)?
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