Transfection Cell Database - Detailed View


Cell Line: HaCat

Cell Line Species/Tissue: human / keratinocyte 
Transfection Reagent: Attractene 
Nucleic Acid:
DNA 
Growth Medium:
DMEM 
Percent Serum (%):
10% 
Reporter System:
GFP 
Plasmid Purification Method:
 
Plate Format:
24-well 
Number of Cells:
4x 10^4 (seeded one day before transfection) 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
0,5 µg 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
2,5 µl 
Complex Incubation on Cells (hrs):
 
Analysis Performed Post-Transfection (hrs):
48 h 
Transfection Efficiency (%):
27% 
 
Any modifications to the protocol?:
 
Notes:
 
References: