| Cell Line Species/Tissue: |
human
/
breast, ductal carcinoma |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
RPMI |
Percent Serum (%):
|
5 |
Reporter System:
|
b-gal |
Plasmid Purification Method:
|
Promega Wizard |
Plate Format:
|
6-well plate |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
2 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
4 - 20 |
Complex Incubation on Cells (hrs):
|
10-12 |
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
20 - 25% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Electroporation gave 10% efficiency. |
| References: |
| |