DNeasy Blood & Tissue Kits

The efficient DNeasy and QIAwave DNA Blood & Tissue Kits procedure enables high yields of total DNA from animal blood and tissue samples (see table Typical DNA yields from animal tissues using DNeasy Blood & Tissue Kits and figure DNA yields). Optimized protocols ensure high yields from nonstandard samples, such as animal hair (see Genotyping of horses), as well as cultured cells, fixed tissues, or Gram-positive and -negative bacteria. Specialized online protocols are available for DNA purification from yeast, insects, hair, and other sample types.

Since the chemistry of the QIAwave DNA Blood & Tissue Kit and the Dneasy Blood & Tissue Kit is identical, they exhibit identical performance. Both kits exhibited superior performance compared to competitor kits (see figure “ QIAwave DNA Blood & Tissue Kit performance”).

Following the standard protocol of the QIAwave DNA Blood & Tissue Kit, high yields of total DNA can be isolated from animal blood and tissue samples (see table "Typical DNA yields from animal tissues using QIAwave DNA Blood & Tissue" and figure "DNA yields"). Furthermore, we also provide optimized protocols to ensure high yields from nonstandard samples, including:

• Animal hair
• Cultured cells
• Gram-positive and -negative bacteria
• Yeast
• Insects
• Other sample types

DNeasy Blood & Tissue Kits simplify purification of DNA from a wide range of sample types, including animal species commonly encountered in life science, veterinary, and genotyping applications (see High-quality DNA). Purified DNA is free from PCR inhibitors, enabling sensitive detection in standard, multiplex (see Efficient 16plex PCR), and real-time PCR (see Real-time PCR). DNeasy Blood & Tissue Kits provide reliable results — from laboratory analysis of transgenic mice (see High-throughput purification) to livestock breeding programs (see Genotyping of pigs) and pedigree genotyping. Routine testing applications can be easily scaled up using the DNeasy 96 Blood & Tissue Kit (see High-throughput purification).

DNeasy and QIAwave DNA Blood & Tissue Kits are designed for rapid purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from a variety of sample sources including fresh or frozen animal tissues and cells, blood, or bacteria.

The DNeasy membrane combines the binding properties of a silica-based membrane with simple microspin technology or with the QIAGEN 96-Well-Plate Centrifugation System. DNA adsorbs to the DNeasy membrane in the presence of high concentrations of chaotropic salt, which remove water from hydrated molecules in solution. Buffer conditions in DNeasy Blood & Tissue procedures are designed to enable specific adsorption of DNA to the silica membrane and optimal removal of contaminants and enzyme inhibitors.

Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. This makes DNeasy Blood & Tissue Kits highly suited for simultaneous processing of multiple samples. For higher-throughput applications, the DNeasy 96 Blood & Tissue Kit enables simultaneous processing of 96 or 192 samples.

Reliable silica-membrane technology, in convenient spin-column or 96-well formats, ensures fast and reproducible DNA purification, eliminating the need for organic extraction and alcohol precipitation (see DNeasy Mini and 96 procedures). Samples are first lysed using proteinase K. Buffering conditions are adjusted to provide optimal DNA-binding conditions and the lysate is loaded onto the DNeasy Mini spin column or the DNeasy 96 plate. During centrifugation, DNA is selectively bound to the DNeasy membrane as contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in two efficient wash steps and DNA is then eluted in water or buffer, ready for use.

DNeasy Mini spin columns in the DNeasy Blood & Tissue Kit are prepackaged in collection tubes and individually sealed, providing convenience and safety. The purification procedure using DNeasy Mini spin columns can be automated on the QIAcube Connect. The DNeasy 96 Blood & Tissue Kit provides high-throughput processing in a 96-well format using the QIAGEN 96-Well-Plate Centrifugation System.

For added convenience, standard protocols for DNeasy Blood & Tissue Kits can be executed seamlessly via the TRACKMAN Connected system, paired with Gilson's PIPETMAN M Connected pipettes. This TRACKMAN Connected system guides researchers through protocols, automatically adjusting Bluetooth-enabled pipette settings. Every protocol step is meticulously recorded, expediting reporting through comprehensive run reports. Download more information.

DNeasy Blood & Tissue Kits and QIAwave DNA Blood & Tissue Kits provide high-quality DNA, ready to use in all downstream assays, including applications in:

• Life science research
• Livestock breeding
• Pedigree genotyping
• Veterinary pathogen research
• Routine applied testing