QIAsymphony DSP Circulating DNA Kits

For automated purification of ccfDNA from 1–192 samples using the QIAsymphony SP

Products

The QIAsymphony DSP Circulating DNA Kits are intended for in vitro diagnostic use.

QIAsymphony DSP Circulating DNA Kit (192)

Cat. No. / ID: 937556

The QIAsymphony DSP Circulating DNA Kit utilizes magnetic-particle technology for automated isolation and purification of human circulating cell-free DNA from biological specimens. Includes Reagent Cartridges, accessories and Proteinase K vials for 192 preps of 2000 µL or 4000 µL or 384 preps of 1000 µL sample volume.

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QIAsymphony DSP Circulating DNA Kit (96)

Cat. No. / ID: 937555

The QIAsymphony DSP Circulating DNA Kit utilizes magnetic-particle technology for automated isolation and purification of human circulating cell-free DNA from biological specimens. Reagent Cartridges, accessories and Proteinase K vials for 96 preps of 2000 µL, 4000 µL, 6000 µL, 8000 µL or 10000 µL or 192 preps of 1000 µL sample volume. For sample volumes larger than 4000 µL, additional Proteinase K vials must be purchased separately.

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QIAsymphony DSP Circulating DNA Maxi Kit (192)

Cat. No. / ID: 937566

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Features

For in vitro diagnostic use
Lot-to-lot traceability
Produced under GMP manufacturing conditions

Product Details

The QIAsymphony DSP Circulating DNA Kits provide reagent cartridges and reagents for fully automated and simultaneous purification of human circulating cell-free (ccf) DNA from human plasma and urine using the QIAsymphony SP instrument. Protocols are available from 1–10 mL sample volumes.

	QIAsymphony DSP Circulating DNA Kit	QIAsymphony DSP Circulating DNA Kit	QIAsymphony DSP Circulating DNA Maxi Kit
Samples	96	192	192
Cat. no.	937555	937556	937566
Number of reactions	96 (2 mL, 4 mL, 6 mL, 8 mL and 10 mL sample volume) 192 (1 mL sample volume)	192 (2 mL and 4 mL sample volume) 384 (1 mL sample volume)	192 (6 mL, 8 mL and 10 mL sample volume)
Reagent cartridge	2	2	2
QIAGEN Proteinase K	3 x 10 mL*	6 x 10 mL	13 x 10 mL

* Additional Proteinase K (cat. no. 19134) bottles should be ordered for 6 mL, 8 mL and 10 mL sample volume to process 96 samples in total.

Principle

QIAsymphony technology combines the speed and efficiency of anion exchange-based nucleic acid purification with the convenient handling of magnetic particles (see figure “ Schematic diagram of the QIAsymphony SP principle.”).

The QIAsymphony SP processes a sample containing magnetic particles as follows: A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod cover is positioned above another well and the magnetic particles are released. These steps are repeated several times during sample processing The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process up to 24 samples simultaneously.

See figures

Efficient processing of magnetic particles.

Procedure

The QIAsymphony SP makes automated sample preparation easy and convenient. Samples, reagents and consumables, and eluates are separated in different drawers. Simply load samples, proteinase K, reagents provided in special cartridges, and preracked consumables in the appropriate drawer before a run. Start the protocol and remove purified DNA from the “Eluate” drawer after processing. Refer to the user manuals supplied with your instrument for operating instructions.

The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 3 steps: bind, wash, and elute (see figure “ QIAsymphony DSP Circulating DNA procedure.”). The user can choose between different sample input volumes.

To process 6–10 mL plasma or urine with the QIAsymphony DSP Circulating DNA Kit (96) (cat. no. 937555), additional Proteinase K (10 mL) (cat. no. 19134). must be purchased.

See figures

QIAsymphony DSP Circulating DNA procedure

Applications

ccfDNA purified using the QIAsymphony DSP Circulating DNA Kit and the QIAsymphony SP is ready for use in a wide range of downstream applications, including NGS, digital, multiplex and quantitative real-time PCR.

Supporting data and figures

Efficient processing of magnetic particles.

The QIAsymphony SP processes a sample containing magnetic particles as follows. A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod with cover is positioned above another well and releases the magnetic particles. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.

Resources

QIAsymphony DSP Circulating DNA Kit Handbook_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

QIAsymphony DSP Circulating DNA Kit Performance Characteristics_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

QIAsymphony DSP Circulating DNA Kit Labware List_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

circDNA_1000_DSP_V2, circDNA_2000_DSP_V4, circDNA_4000_DSP_V4, circDNA_6000_DSP_V1, circDNA_8000_DSP_V1, and circDNA_10000_DSP_V1
QIAsymphony DSP Circulating DNA Kit Protocol Sheet_V2_In Vitro Diagnostic use according to the Regulation (EU) 2017/746 on in vitro diagnostics medical devices

Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.