| Cell Line Species/Tissue: |
Rat
/
Liver |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM, 400 µg/ml G418 |
Percent Serum (%):
|
|
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well |
Number of Cells:
|
1×104 |
Percent Confluence(%):
|
70 % |
Amount of Nucleic Acid (µg):
|
1 µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
no information |
Complex Incubation on Cells (hrs):
|
|
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
|
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| Inhibition on the production of collagen type I, III of activated hepatic stellate cells by antisense TIMP-1 recombinant plasmid
Wen-Bin Liu, Chang-Qing Yang, Wei Jiang, Yi-Qing Wang, Jing-Sheng Guo, Bo-Ming He, Ji-Yao Wang
World J Gastroenterol 2003;9(2):316-319 |