| Cell Line Species/Tissue: |
Human
/
Retina |
| Transfection Reagent: |
RNAiFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM-Ham's F12-Medium; 100 U/ml Penicilin, 100µg/ml Streptomycin, 2mM Glu |
Percent Serum (%):
|
2% FCS |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
6-well |
Number of Cells:
|
1.5x10e5 |
Percent Confluence(%):
|
50% |
Amount of Nucleic Acid (µg):
|
5µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
15µl or 30µl |
Complex Incubation on Cells (hrs):
|
6h |
Analysis Performed Post-Transfection (hrs):
|
24h, 48h, 72h |
Transfection Efficiency (%):
|
N.D. |
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| no |
| Notes: |
| |
| References: |
| |