| Cell Line Species/Tissue: |
/
|
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
5 |
Reporter System:
|
GFP |
Plasmid Purification Method:
|
QIAGEN Plasmid Kit |
Plate Format:
|
75cm^2 |
Number of Cells:
|
|
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
3,47 |
Amount of Enhancer (µl):
|
27,76 |
Amount of Reagent (µl):
|
86,7 |
Complex Incubation on Cells (hrs):
|
16h |
Analysis Performed Post-Transfection (hrs):
|
40h |
Transfection Efficiency (%):
|
70-80 clones/10000 cells |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |