Transfection Cell Database - Detailed View


Cell Line: C57

Cell Line Species/Tissue: Mouse / embryonic fibroblasts  
Transfection Reagent: SuperFect 
Nucleic Acid:
DNA 
Growth Medium:
 
Percent Serum (%):
10 
Reporter System:
GFP 
Plasmid Purification Method:
QIAfilter Plasmid Kit 
Plate Format:
100-mm dish 
Number of Cells:
 
Percent Confluence(%):
40-60 
Amount of Nucleic Acid (µg):
15 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
22.5 
Complex Incubation on Cells (hrs):
3.5 
Analysis Performed Post-Transfection (hrs):
24 
Transfection Efficiency (%):
5-15 
 
Any modifications to the protocol?:
 
Notes:
 
References: